Single nucleotide polymorphisms in the growth hormone and insulin-like growth factor-1 genes are associated with milk production, body condition score and fertility traits in dairy cows

Single nucleotide polymorphisms (SNPs) provide a highly useful indicator for livestock production. In this study, SNPs in bovine growth hormone (GH1) and insulin-like growth factor 1 (IGF-1) genes were studied to quantify their association with direct performance trait measurements of lactation and fertility in Holstein-Friesian lactating dairy cows. Sixteen SNPs in the IGF-1 and GH1 genes were genotyped in 610 dairy cows using iPLEX® Gold assays and analyzed for their association with different performance traits.

Study Highlights

• 1 SNP in IGF-1 was associated with increased milk yield, fat yield, and protein yield or body condition score at calving
• 1 SNP in GH1 was associated with milk yield and 3 SNPs were associated with pregnancy and calving traits
• 1 SNP in GH1 may be associated with the modulation of a transcription factor binding site

Association mapping of common bacterial blight resistance QTL in Ontario bean breeding populations

Common bacterial blight (CBB) is a major problem in common bean production, and host resistance is the most effective approach for its control. In the study led by Shi et al, SNP genotyping-based association mapping was evaluated to determine whether it could be used to effectively discover resistance quantitative trait loci (QTLs). The MassARRAY® system was used to design >100 SNPs evenly distributed across 11 bean chromosomes. Over 460 lines were analyzed with >80% call rate. CBB resistance was evaluated 14 and 21 days after inoculation. The group ultimately determined that association mapping using SNPs distributed across the genome can be used to detect QTLs in plant breeding.

Study Highlights

• 18 markers were associated with resistance after 14 days and 22 with resistance after 21 days
• 14 markers were associated with resistance after 14- and 21 days
• 12 markers were co-localized with previously identified CBB-QTLs

Upcoming Events:
Visit us at the International Plant & Animal Genome XX / January 14-18, 2012 — San Diego, CA Booth #:103

Come to our workshop
Time: Pacific Salon 3
Room: Tuesday, January 17, 2012
Details:
Targeted Genotyping Solutions for Plant and Animal Genomics

Sequenom’s MassARRAY® system provides a highly flexible and cost-effective solution for SNP panel development for marker validation and high-throughput sample screening. In this workshop, relevant applications for livestock and polyploidy crop testing will be provided.

1:30 PM - Applications of Automated DNA Mass Spectrometry in Agricultural Genomics - Divya Neelam, Sequenom 1:50 PM - Detection of QTL Using an Economical Hybrid Genotyping Approach - Gary Rohrer, USDA-ARS
2:10 PM- Quantitative Genetic Analysis of Polyploid Plant Species using MassARRAY - Anders Nygren, Sequenom