Agricultural Genomics

Useful Links 

• MassARRAY for Agricultural Genomics Brochure
• Bovine Plant Genomics Presentation

MassARRAY System – Flexible and Cost-Effective High-throughput Screening Tool for Agricultural Research

Genome-wide association and next-generation sequencing studies have identified a vast number of genetic markers, which are now being used in molecular breeding of plants and animals for a variety of improvements, including increased yields, disease resistance, feed efficiency and nutrition.  With its unmatched performance and versatility, Sequenom’s MassARRAY^® system is a powerful tool for downstream marker validation and large-scale screening.

Product Spotlight for Agricultural Genomics

• Specially designed High Throughput Genotyping Reagent Set for agricultural applications requiring  large number of samples on regular basis
• Highly flexible system: Cover all of your important SNPs. No need to limit your panel to a fixed SNP number.
• Modifying an existing panel is fast and easy
• Low cost per SNP and short reagent order lead time
• High sample throughput: 10 hours from DNA to results with 65 minutes hands-on-time.  One technician can generate 4,000-230,000 genotypes on one MassARRAY system per day.
• High call rate irrespective of organism: Up to 99% call rate on agricultural organisms.
• Applications beyond genotyping: In addition to SNP genotyping, the MassARRAY platform also supports copy number variation, gene expression, and methylation applications.

Workshop Recording: Sequenom workshop at Plant and Animal Genome XIV
Targeted Genotyping Solutions for Large Scale Plant and Animal Genomics:
Dr. Bernhard Benkel, Nova Scotia Agricultural College*
Dr. Wing Cheung, DNA Landmarks, a BASF Plant Science company*
Divya Neelam presented their work on bovine, tomato, wheat and canola genotyping on Sequenom MassARRAY platform.*

Dr. Miri-Cohen Zinder from University of Illinois at Urbana-Champaign, Professor Robert Henry from Southern Cross University, and Dr. Adam Shahid from Pfizer Animal Genetics presented their studies on livestock and polyploid crop genetic testing.

Dr. Richard Buggs describes how Sequenom technology has been used to validate SNPs called from next-generation sequence data, and detect those SNPs in a large number of non-sequenced individuals. He also discusses how relative expression of homeo-alleles can be measured using Sequenom allelotyping.

Dr. Patrick S. Schnable, Baker Professor of Agronomy from Iowa State University, presents "High-throughput genetic mapping of mutants via quantitative SNP-typing" using the Sequenom MassARRAY system. Dr. Schnable discusses how to convert putative SNPs discovered by next gen sequencing into genetic markers that can be scored reliably and used to generate a SNP-based maize genetic map. He also shares his experiences in developing a strategy to rapidly map the loci associated with recessive mutants, taking advantage of the quantitative nature of the Sequenom-based SNP assays.