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EpiTYPER® and iSEQ™ 96 Applications on MassARRAY® Analyzer 4
Coming soon
MassARRAY Analyzer 4 – 96-well system – is specifically designed to address the needs of low to mid-throughput labs.
The system now offers:
- EpiTYPER software for quantitative DNA methylation analysis
- iSEQ software for comparative sequence analysis
- Two-chip MassCLEAVE™ Reagent Sets to accommodate small studies
Featured Publications
Combining QTL mapping and transcriptome profiling of bulked RILs for identification of functional polymorphism for salt tolerance genes in rice
Rice is a staple food for more than half of the world's population. Understanding the complex relationship between soil, water, and plant genotype is key to developing salt-tolerant rice varieties for the affect areas. Identification of the functional polymorphism of genes for different salt tolerance parameters was established by combining genetic mapping and transcriptome profiling of bulked RILs with extreme phenotypes. The Sequenom MALDI-TOF MassARRAY system offered a robust method for genotyping 88 SNPs, including 72 genome wide SNPs from conserved single copy rice genes, and a set of 16 SNPs identified by re-sequencing of candidate genes.
A genome-wide association study using selective DNA pooling identifies candidate markers for fertility in Holstein cattle
While milk production by dairy cattle has increased over the last few decades, fertility of cows has shown a dramatic decrease over the same time. A recent publication in the December 2010 issue of Animal Genetics describes the use of selective DNA pooling-based GWAS to identify genetic markers associated with IVF fertility traits. Fertilization and blastocyst rates for 496 Holstein cows and 12 bulls were recorded and DNA pools constructed from the upper and lower 15-20% of the phenotypic distributions. After mapping, SNPs that reached 5% genome-wide significance (27 in total) were genotyped for all individual DNA samples in the resource population using Sequenom iPLEX genotyping assays. The majority of SNP associations could be reproduced (13 SNPs had P-values <0.05) testifying to the effectiveness of selective DNA pooling using a high-density genotyping array with follow-up validation.
High concordance of bovine single nucleotide polymorphism genotypes generated using two independent genotyping strategie
Accurate and reliable low-medium genotyping platforms are required to make correct decisions in animal management and breeding programs. In this study, 263 Bos spp. samples were genotyped for a total of 16 selected SNPs using Sequenom iPLEX Gold assays and validated with Taqman and bi-directional sequencing technologies. The call rate was 99.31% (SD ±0.93%). The concordance of all 3,744 duplicate genotypes in iPLEX Gold and Taqman techonologies was 99.92%, which suggests both methods generate high SNP genotype call rates and the data support the use of either method especially for candidate gene-phenotype association studies.
Allelic variation in cell wall candidate genes affecting solid wood properties in natural populations and land races of Pinus radiate
Phenotypic properties for the pine tree, Pinus Radiata, a commercially important tree species, vary widely and these variations are commonly controlled by inherited genetic variations. Dillon et al, working in Australia, performed a series of experiments to determine whether SNP's could be found which lead to desirable wood quality and growth characteristics. Of 149 haplotype tagging SNPs investigated, the researchers discovered 25 SNPs demonstrating potential correlation with wood quality and growth using Illumina Universal Bead Arrays. These candidate SNPs were validated using the Sequenom technology on a population of 458 individuals. The researchers discovered 3 genes that showed strong correlation with desirable wood quality and growth. By utilizing the Sequenom technology, the authors were able to readily validate the effects of candidate SNPs, potentially leading to a genetic test that can be used to assist in breeding efforts.
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Upcoming Events:
Name: PAG Annual Meeting
Date: Jan. 15-19, 2011
Details: Come visit us at booth # 107
Name: PAG workshop
Date: Jan. 17, 2011 at 6:10 PM
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