EpiTYPER® Tools for DNA Methylation Analysis
Quantitative and Reproducible DNA Methylation Profiling
Sequenom’s EpiTYPER platform measures DNA methylation patterns precisely and accurately by combining bisulfite treatment with the trusted MassCLEAVE™ chemistry and MassARRAY® MALDI-TOF MS platform used in genotyping labs worldwide. It is the ideal solution for post-array and deep sequencing validation or targeted gene promoter analysis. This carefully optimized research workflow provides:
Accurate and Reproducible Methylation Mapping
- Delivers individual methylation ratios for CpGs within a target sequence
- Relative methylation ratios can be assessed in a range between
10-90% with a standard deviation of 5%
Flexibility
- Scalable for investigating a few or several hundred regions over multiple samples
- Long reads up to 600 bp in one reaction enable discovery of differential methylation within large promoter regions
- Robust analysis from a range of sample types including Formalin Fixed Parraffin Embedded (FFPE) samples
Convenience
- Chose from pre-validated assays in the EpiTYPER Panels
- Design custom assays with the freely-available EpiDesigner Program
- Send your samples to Sequenom for EpiTYPER Methylation Analysis Services
Validated Methylation Assays with EpiTYPER EpiPanels
EpiTYPER technology, combined with the simplicity of the following pre-designed panels, offers rapid and quantitative methylation profiling of genes frequently associated with disease and developmental states. Our current EpiPanels include:
Standard EpiPanel: This panel includes pre-validated assays for 41 frequently studied genes. The panel offers the speed and convenience of using “off the shelf” validated assays to simplify and standardize methylation analysis.
Cancer EpiPanel: The Cancer EpiPanel contains targets more than 400 cancer related genes. It includes pre-validated assays covering over 12,000 CpG sites in promoter regions of genes known to be involved in neoplastic transformation and imprinting. The Cancer EpiPanel was used to produce quantitative DNA methylation profiles for more than 400 cancer related genes over 59 cell lines (NCI60) and normal tissues derived from 9 different tissue types (Ehrich et al. PNAS 2008).
EpiBrowser is a user-friendly tool that enables you to quickly browse the database and display genes/samples of interest quickly and logically in charts, tables, heatmaps and epigrams. The database not only provides information on the methylation status of CpG sites throughout the cancer related panel, but also provides validated primer sequences for amplifying the regions of interest that our team has interrogated. These validated primer sequences are available for download free of charge to facilitate your methylation studies.
Imprinting EpiPanel: This panel includes 31 pre-validated assays for the most commonly studied genes from which the methylation pattern is either paternally or maternally derived (Jirtle and Weidman, 2007).
Custom Methylation Assays with EpiDesigner Software
EpiDesigner is a free primer design tool available at www.epidesigner.com for designing optimal primer sets for amplifying genomic regions of interest in quantitative methylation analysis studies. If your gene(s) of interest are not included in the Sequenom EpiPanels, you can easily design assays for any genomic region using Sequenom’s EpiDesigner software. Go to www.epidesigner.com today and see how EpiDesigner will facilitate your methylation studies.
EpiTYPER Methylation Analysis Services
If you don’t have the time or you don’t have access to a MassARRAY® system, send your purified DNA samples to Sequenom’s Services group and we will handle your experiments for you. Our highly trained scientists will consult with you to design an experiment that will be sure to meet your experimental goals.
Inquire today and let us help you design your next experiment.
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