How it Works

Biochemistry Overview

Total RNA or mRNA is reverse-transcribed to cDNA. The resultant cDNA and a synthesized competitor that differs in one nucleotide undergo real competitive PCR (rcPCR).

Following amplification, remaining nucleotides are deactivated by SAP treatment (not shown). A single base primer extension step is performed, and the primer extension products are quantitatively analyzed using MALDI TOF MS.