OncoCarta™ Panel v1.0

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» Product Description
» Mutation List
» Protocol
» Product Info
» Kit Components
» Intended Use
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Rare Mutations Profiling in Cancer

Recent evidence has indicated that rare genetics variants may play a significant role in tumorigenesis, pathogenesis, and disease progression¹. Genetic mutations resulting in the activation of oncogenes increase the probability that a normal cell will develop into a tumor cell. The frequency of oncogene mutations across human tumor types has been investigated in previous studies—30% of 1,000 tumor samples carried at least one mutation², but further research can lead to oncogene mutation maps for specific tumor types, diseases, and/or individuals.

Until recently, profiling such genetic variants across many individuals with the necessary accuracy and coverage was not possible because either SNP arrays provide a whole-genome association view, or sequencing interrogates a specific region or locus. The Oncocarta™ Panel addresses these limitations, enabling investigation of over 230 mutations in parallel for fine mapping of single and co-occurring events across multiple samples.

Product Description

The OncoCarta™ Panel v1.0 provides a highly sensitive group of assays for oncogene detection.

• Pre-designed assays for profiling multiple mutations in challenging genes such as EGFR, BRAF, KRAS, and KIT , eliminating any need for design optimization
• Thorough coverage with over 230 mutations across 19 oncogenes, providing an in depth look at the key mutations
• Ability to detect and quantify as low as 10% mutation frequency for low abundance events, enabling you to profile variants previously not possible with greater accuracy

Protocol

PCR amplification and primer extension are performed using the OncoCarta™ Panel reagents to detect target molecules by MALDI-TOF mass spectrometry. Users run the entire panel consisting of 24 multiplexes per sample using a total of 480 ng. DNA from either cells, fresh, frozen or paraffin-embedded tissue. Following the sample run, you can detect and quantify mutation levels simultaneously across samples. Up to 32 samples (2 x 384-spot chips) can be processed in a single day, with time to analysis <1 hour using new enhanced Typer^® software.

Product Info

The OncoCarta™ Panel v1.0 kit components include reagents sufficient for processing 160 samples.

Kit Components

• PCR Reagents: 24 PCR Primer Mixes, PCR Reagent Set for amplification
• Extension Reagents: 24 PCR Extend Mixes, Extension Reagent Set
• SpectroCHIP^® II and Clean Resin
• TyperAnalyzer software add-on

Intended Use

The OncoCarta™ Panel v1.0 is intended for research use only, and not for use in diagnostic procedures.

References

1. Cosmic database
2. Thomas et al, Nature Genetics, Volume 39, Number 3, March 2007

Early Access