Technical Information Downloads
DNA Methylation Software Tools
- EpiDesigner: Primer Design Software for Bisulfite Converted DNA
The EpiTYPER™ assay is based on proven bisulfite conversion biochemistry, followed by PCR and a proprietary basespecific cleavage process. The resulting cleavage pattern depends on the presence of methylated cytosine in the original genomic DNA. The cleavage products are automatically and quantitatively analyzed by MALDI-TOF mass spectrometry.
MALDI TOF MS analysis of the cleavage product results in a distinct signal pattern from the methylated and non-methylated template DNA.
Genomic DNA undergoes bisulfite treatment followed by PCR amplification in which a T7 promoter tag is introduced. Next, in vitro RNA transcription is performed on the reverse strand, followed by a base specific cleavage. |