How it Works
Figure 2. Plate Editor software interface
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iSEQ™ Software Overview
The iSEQ™ software suite enables rapid, efficient plate design, automatic data analysis, and sample identification. The Plate Editor enables you to custom design and store your microplate formats with your samples, reference sequences, and reactions. All plate formats are automatically stored in the database for later retrieval. The Plate Editor software interface is depicted in Figure 2.
During analysis, the experimental mass pattern of the sample is automatically compared to an in silico reference mass signal pattern derived from an individual or a set of reference sequences. Differences between the expected and the observed mass signal pattern are interpreted and enable identification of the organism and detection of any sequence variation.
Identification results are automatically retrieved in less than an hour. The iSEQ software generates a report containing ID results listing the best matching reference sequences for each target region of interest, as well as, sequence variations and new sequences. A graphical software interface of the ID results and mass spectra is provided in Figure 3.
Figure 3. Data Analysis software interface
In addition to the iSEQ software, we have developed a Simulation Software to enable the user to simulate peak patterns for reference sequences and to determine the optimal number of reactions to run. To download the latest version of the simulation tool, click the CONTACT tab at the top to register. |