Genetic Analysis
Molecular Typing
Applications Applications
DNA Methylation Analysis DNA Methylation Analysis
SNP Genotyping SNP Genotyping
Molecular Typing Molecular Typing
iSEQ Overview iSEQ Overview
How it Works How it Works
Reagents Reagents
Literature Literature
Rare Mutation Profiling Rare Mutation Profiling
Gene Expression Analysis Gene Expression Analysis
Research Area Research Area
Systems Systems

Reagents

Reagents

The MassCLEAVE™ chemistry has been optimized to provide the highest quality of data for EpiTYPER®, SNP Discovery, and iSEQ™.
MassCLEAVE is based on an in vitro transcription ribonuclease enzymatic cleavage of 500 – 800 bp PCR products of the target region of interest.
The MassCLEAVE process is homogeneous and does not require purification of the PCR product or subsequent analytes.


The kit contains sufficient reagent amounts to process ten 384 well microplates. The workflow is automatable and thus amenable to high throughput. To ensure success, PCR reagents are also available for amplification of the target region of interest.

Ordering Information

Homogeneous MassCLEAVE™ (hMC) Consumables
Catalog No. Item Description
10149 MassCLEAVE™ Kit - T7 / SP6
(T - and C - Cleavage)
Recommended for  iSEQ
10129.1 MassCLEAVE™ Kit - T7
(T - Cleavage)
Standard for EpiTYPER, optional for iSEQ
10129.2

MassCLEAVE™ Kit - T7
(C - Cleavage)  Optional for iSEQ

PCR Consumables
Catalog No. Item Description
11327 PCR Accessory Set (3840 reactions):
·  Buffer
·  MgCl2
·  dNTP
01462 PCR Enzyme (3840 reactions):
·  5000 units, 5U/ ml
11324 Complete PCR Reagent Set (3840 reactions):
  ·  PCR Accessory Set
·  PCR Enzyme
10148 Complete Genotyping Reagent Set (3840 reactions):
  ·  Complete PCR Reagent Set
Spectro CHIP® Arrays and Clean Resin Kit
Catalog No. Item Description
10117 Spectro CHIP® Arrays and Clean Resin Kit
·  Chips (Box of 10)
·  Clean Resin 28g for 10 Chips