Genetic Analysis
Gene Expression Analysis
Applications Applications
DNA Methylation Analysis DNA Methylation Analysis
SNP Genotyping SNP Genotyping
Molecular Typing Molecular Typing
Rare Mutation Profiling Rare Mutation Profiling
Gene Expression Analysis Gene Expression Analysis
QGE Overview QGE Overview
How it Works How it Works
Reagents Reagents
Literature Literature
Research Area Research Area
Systems Systems

Reagents for QGE Experiments

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Sequenom now offers a newly developed method, the iPLEX® Gold Assay for primer extension. The iPLEX® Gold reaction is a universal method for detecting insertions, deletions, substitutions, and other polymorphisms in amplified DNA, and is commonly used in SNP genotyping assays. For QGE, the competitor acts as an artificial allele resulting into two different extension products for each gene of interest under evaluation (see example in figure below).

QGE Reagents Fig.1

iPLEX Reaction
  1. Prepare the iPLEX® Gold reaction cocktail
  2. Dispense 10 ml iPLEX® Gold cocktail into each well of a 96-well plate
  3. Robotically transfer 2 ml iPLEX® Gold cocktail from the 96-well plate to a 384-well plate
  4. Cycle as follows:

QGE Reagents Fig.2