Genetic Analysis
DNA Methylation Analysis
Applications Applications
DNA Methylation Analysis DNA Methylation Analysis
Pre-Validated DNA Methylation Assays Pre-Validated DNA Methylation Assays
Custom DNA Methylation Assays Custom DNA Methylation Assays
SNP Genotyping SNP Genotyping
Molecular Typing Molecular Typing
Rare Mutation Profiling Rare Mutation Profiling
Gene Expression Analysis Gene Expression Analysis
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Systems Systems

EpiTYPER DNA Methylation Analysis Protocol Overview

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DNA Methylation Software Tools
  • EpiDesigner: Primer Design Software for Bisulfite Converted DNA
EpiTYPER - How it Works

The EpiTYPER™ assay is based on proven bisulfite conversion biochemistry, followed by PCR and a proprietary basespecific cleavage process. The resulting cleavage pattern depends on the presence of methylated cytosine in the original genomic DNA. The cleavage products are automatically and quantitatively analyzed by MALDI-TOF mass spectrometry.

 

MALDI TOF MS analysis of the cleavage product results in a distinct signal pattern from the methylated and non-methylated template DNA.

EpiTYPER Biochemistry Overview

Genomic DNA undergoes bisulfite treatment followed by PCR amplification in which a T7 promoter tag is introduced. Next, in vitro RNA transcription is performed on the reverse strand, followed by a base specific cleavage.