How it Works
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EpiTYPER Biochemistry Overview
Genomic DNA undergoes bisulfite treatment followed by PCR amplification in which a T7 promoter tag is introduced. Next, in vitro RNA transcription is performed on the reverse strand, followed by a base specific cleavage.
MALDI TOF MS analysis of the cleavage product results in a distinct signal pattern from the methylated and non-methylated template DNA.
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